Research Technician V

CancerCare Manitoba is a provincial agency which provides clinical and academic services and leadership for cancer control and the treatment of blood disorders. Our shared vision of a world free of cancer drives every member of our team. CancerCare Manitoba is the perfect place to thrive if you're seeking a fulfilling career in an organization that is committed to its employees' well-being and professional development. Competition # 350-24-761069505 Posting End Date: Ongoing City: Winnipeg Work Location: MacCharles Department: Paul Albrechtsen Research Institute Union: Non-Union Hiring Status: Permanent Anticipated Start Date: ASAP FTE: 1.0Anticipated Shift: Days Daily Hours Worked: 7.50 Annual Base Hours: 1950 Salary: $28.460, $29.650, $30.860, $32.150, $33.550, $34.950, $, $36.350, $37.800, $39.320, $40.880 Job Summary: The CancerCare Manitoba Research Institute (CCMR) through the support of the CancerCare Manitoba Foundation is developing an innovative, single cell technologies (SCT) research platform that requires a Platform Manager, a Platform Technician and a CRISPR Technician. The SCT Platform will include state-of-the-art instrumentation enabling: 1) the isolation of single cells (BD Bioscience, FACSMelody); 2) the characterization of single cells (10X Genomics, Chromium Connect and X); 3) the quantitative phenotyping of single cells (Molecular Devices, ImageXpress HT.ai; Akoya Biosciences, PhenoCycler Fusion; BioTechne ProteinSimple, Jess); and 4) Cancer modelling (CRISPR Cas9 library). Reporting directly to the Director of the Platform, the CRISPR technician will work with researchers to design, generate and organize human and mouse CRISPR libraries; perform molecular cloning/sub-cloning of individual CRISPR vectors; perform in silico analyses/sequence retrieval for CRISPR sgRNA design; prepare and send samples for DNA sequencing; retrieve, grow and store plasmid stocks; generate viral particles and CRISPR-modified cell lines; provide training; assist with generation of novel CRISPR models (CRISPR knockout; gene modification; CRISPRa/i); participate in the Advisory User Committee meetings; and, seek out/attend continuing education and professional development opportunities to maintain up-to-date knowledge of approaches. Qualifications:Required:

• BSc or MSc in Science, preferably in life sciences, a cancer-related field or relevant scientific discipline
• Experience with Microsoft Office suite (Word; PowerPoint; Excel)
• Experience working with multi-disciplinary research teams
• Experience preparing scientific presentations/literature
• Must hold a satisfactory employment record
• Must be able to legally work in Canada

Will be assessed during the interview process

• Strong organizational skills, exceptional attention to detail with the ability to work independently and prioritize work to meet deadlines and meet milestones
• Excellent interpersonal, verbal and written communication skills
• Ability to work quickly, accurately and with decorum while under deadline pressure and continuous interruptions
• Willingness to learn and share (train) new techniques/technologies
• Ability to learn quickly and work on own initiative (i.e., highly motivated and self-starter)
• Ability to safeguard confidentiality
• Project and time management skills

Preferred:

• Knowledge and hands-on experience with gene editing technologies (e.g., CRISPR/Cas9) and their application in research
• Prior knowledge/experience with molecular biology techniques including but not limited to molecular cloning/sub-cloning, primer design, bacterial transformation and plating, PCR, gel electrophoresis and DNA sequencing
• Experience with cell culture and virus (e.g., lentivirus, retrovirus, adenovirus) production
• Effective communication skills with diverse audiences

Asset:

• Ability to speak French

Interested and qualified applicants are invited to submit a detailed and current resume, plus a cover letter demonstrating how you meet the above qualifications and the above competition number. This position is subject to a Criminal Record Check, including Vulnerable Sector Search, an Adult Abuse Registry Check and a Child Abuse Registry Check. The successful candidate will be responsible for any service charges incurred. CancerCare Manitoba is committed to developing inclusive, barrier-free recruitment processes and work environment. If you are contacted by CancerCare Manitoba regarding a job opportunity or testing, please advise if accommodation measures must be taken to enable you to apply and be assessed in a fair and equitable manner. We thank all that apply and advise that only those applicants selected for further consideration will be contacted.